Plants have been an important source of medicine since ancient era. Gcms analysis of chemical compounds from some yemeni medicinal plants. Thus, this type of study may give information on nature of active principles present in the medicinal plants. Comparison of the proximate composition, vitamins ascorbic. The presence of new minor constituents is usually inferred from gas chromatography mass spectrometry gc ms analyses that provide only a mass spectrum and retention data, which are insufficient. Gcms analysis of the essential oil and methanol extract of. Interpretation on mass spectrum gcms was conducted using the database of national institute standard and technology nist which is having more than 62,000 patterns. The concentrated extract was subjected to gcms analysis to detect the phyto constituents. The constituents of the nhexane extract and fractions were characterized and identified by gcms. A study of phytochemical constituents in caralluma umbellata. Gas chromatographymass spectroscopic analysis of evolvulus alsinoides.
Gcms analysis of leaf ethanolic extracts of wrightia. Recently gc ms analysis is increasingly applied for the analysis of medicinal plants and this technique prove to be a valuable method for the analysis of nonpolar components and volatile essesntial oil, fattyacids, lipids and alkaloids4. The most important of these bioactive compounds of plants are alkaloids, flavanoids, tannins and phenolic compounds h. The present study deals with the phytochemical and gc ms analysis of the various leaf extracts of one such medicinal plant, carissa spinarum l. Phytochemical, cordia dichotoma leaves, gcms analysis introduction the search for natural products to cure diseases represents an area of great interest in which plants have been the most important source. The rhizomes of the plant nervilia aragoana were collected, washed. Paper open access qualitative analysis, total phenolic. Bachrachcontribution of selected medicinal plants for cancer prevention.
Phytochemical analysis phytochemical screening of crude extracts of pergularia daemia was carried out according to the methods described by trease and evans 15,16. The detailed tabulations of gcms analysis of the extracts are given in table 1, table 2, table 3, table 4 and table 5 respectively. These identified phytoconstituents presumed to be responsible for eliciting the traditional activity of this plant justicia adhatoda. Pdf the gc ms analysis of one medicinal plant, premna tomentosa. Gcms determination of organochlorine pesticides in five medicinal plants. Sanghamitra nayak, alok kumar jena, deepak kumar mittal, deepmala joshiand, gcms analysis of phytocostituents of some wild. Gcms analysis acetone extract of acalypha indica leaves was analyzed with the help of gcms analyzer gc clarius 500 perkin elmer. Gcms analysis of bioactive phytochemicals present in ethanolic. Medicinal plants are at great interest to the researcher in the field of biotechnology, as most of the drug industries depend in medicinal plants for the production of pharmaceutical compounds. Pdf phytochemical analysis of leaf extract of eclipta. This study forms a basis for the biological characterization and importance of the compounds identified and creates a platform to screen many bioactive components to treat many diseases.
Studies on the medicinal plant acalypha wilkesiana ethanol. Gcms analysis of nhexane and chloroform extracts of. Gcms analysis of ethyl acetate extract of whole plant of. Gc ms determination of organochlorine pesticides in five medicinal plants. Gcms ionizes compounds and measures their mass numbers. Gcms is normally used for direct analysis of components existing in traditional medicines and medicinal plants. Phytochemical analysis was carried out for identification of tannins, terpenoids, flavonoid, alkaloid, phenol, phytosterol and saponins according to standard methods trease and evans, 2008. Plants are the traditional sources for many chemicals used as pharmaceutical biochemicals, fragrances, food colours and flavours in different countries especially in india. Phytochemical screening and gcms analysis of mukia. Gcms analysis methanol extract of solanum torvum leaves was analyzed with the help of gcms analyzer perkin. Gcms analysis of phytocomponents in the ethanol extract of. Gcms analysis of the essential oil and methanol extract. Gcms analysis of phytocostituents of some wild zingiberaceae. Volatile compounds are identified by the gc ms analysis3.
Gcms analysis of bioactive constituents of indigofera. With this background the present study was aimed to identify the phytoconstituents in indigofera suffruticosa leaves by using gcms analysis. Phytochemical screening and gcms, ftir analysis of. Gcms analysis of chemical compounds from some yemeni. There are many reports on the phytochemical and gc ms analysis of plants and plant parts and there medicinal formulations 12345678 9 10. Introduction the indian subcontinent is rich in medicinal plants and is one of the richest countries in terms of genetic diversity of medicinal plants. Gcms analysis of bioactive compounds from the whole plant.
Phytochemical screening and gcms analysis of acetone. Gas chromatography gcms is normally used for direct analysis of components existing in traditional medicines and medicinal plants. Gc ms analysis of chemical compounds from some yemeni medicinal plants. India is the largest producer of medicinal herbs and is appropriately called the botanical garden of the world. One approach commonly used for weed control is chemical herbicides. Eclipta alba has an important role in the traditional ayurvedic, siddha and unani systems of medicine. Gcms analysis of volatile constituents some extracted from. Pandiyarajan pg and research department of botany, h. In recent years gcms studies have been increasingly applied for the analysis of medicinal plants as this technique has proved to be a reliable method for the analysis of nonpolar components and volatile essential oil, fatty acids, lipids and alkaloids 89. The present study deals with the gc ms analysis of a medicinal plant premna tomentosa which is known as an important medicinal plant. Calotropis gigantea, phytochemical screening, gcms analysis.
Gcms analysis of bioactive compounds in methanolic. The gc ms analysis of one medicinal plant, premna tomentosa. Phytochemical analysis of hybanthus enneaspermus using. Analysis of herbicidal activity from medicinal plants by gcms and seed bioassay of weeds. Gcms analysis and phytochemical screening of a rare. Out of 46 compounds, 24 were identified from leaves. Research article gcms analysis of phytochemical compounds present in the rhizomes of nervilia aragoana gaud elizabeth thomas1, aneesh t. The gcms analysis of bioactive compounds from the different extracts of the. In recent years gc ms studies have been increasingly applied for the analysis of medicinal plants as this technique has proved to be a reliable method for the analysis of nonpolar components and volatile essential oil, fatty acids, lipids and alkaloids 89. Gcms analysis the fatty acid composition of any plant extract determines all the chemical properties by using the gcms of the fatty acid methyl esters yeboaha et al. In the present study, total 46 compounds were identified from the leaf and latex extract of c. The medicinal value of these plants lies in some chemical substances that produce a definite physiological action on the human body.
Calotropis gigantea, phytochemical screening, gc ms analysis. The aim of the present study is to determine the potential bioactive components of leaves of eclipta alba using gas chromatography mass spectrometry analysis gcms. There are numerous reports on the gc ms analysis studies on many plants and plant parts. Pdf characterization of some plant extracts by gcms. The equipment has a db 35 ms capillary standard nonpolar column with dimensions of 30 mm. Gcms analysis of leaf ethanolic extracts of wrightia tinctoria a high medicinal value plant t. The use of medicinal plants as traditional medicines is well known in. Alhajj1,abdullbaky alzaemy2, hongxin wang3, chaoyang ma3,zaixiang lou3, riyadh thabit3 1. Phytochemical, gcms analysis and antibacterial activity of a. It exhibits a wide range in topography and climate. The gcms analysis of the essential oil of the seeds identified the presence of.
Phytochemical analysis of crude powder of the samples for the evaluation of phytochemicals. Compounds were separated on elite 5ms 5% diphenyl 95% dimethyl polysiloxane, 30x0. These studies were undertaken to ascertain the presence of active bio molecules which have therapeutic activities 5, 6. Gcms analysis of the whole plant extract of evolvulus alsinoides was performed using the equipment thermo gctrace ultra version. Gcms analysis of ethyl acetate extract of whole plant of rostellularia diffusa. Thus this type of gcms analysis is the first step towards understanding the nature of active principles in this medicinal plant and this type of study will helpful for further detailed study. Qualitative analysis, total phenolic content, ftir and gcms characterisation of canna indica.
Phytochemical analysis and assessment of antioxidant and. Plants 2019, 8, 51 2 of 15 morris 2011 4, attests that the use of medicinal plants is the most widespread cultural heritage and the most ancient form of treatment. Recently gcms analysis is increasingly applied for the analysis of medicinal plants and this technique prove to be a valuable method for the analysis of nonpolar components and volatile essesntial oil. Gas chromatographymass spectrometry gcms is a robust approach for the qualitative and quantitative analysis of metabolites of plant origin iordache et al. Gcms analysis of chemical compounds from some yemeni medicinal plants nabil qaid m. Journal of medicinal plants studies gcms determination of. C, at split ratio of 550 using db5mscolumn 30m x 0. Most of the active compounds have identical name or synonym name which is important for searching their function and application. Procedure for gcms analysis the nhexane extracts obtained from the heartwood and stem bark of a. Gcms analysis of bioactive compounds present in different. Gcms analysis of chloroform extract of cassia fistula leaves table 3. However, the absence of scientific evaluation of medicinal plants may cause serious adverse effects. Spectroscopic detection by gcms involved an electron ionization system which utilized high energy electrons 70 ev. Jul 24, 20 gcms analysis of the whole plant extract of evolvulus alsinoides was performed using the equipment thermo gctrace ultra version.
Anandan3 1amrita school of pharmacy, amrita vishwa vidyapeetham university, aims health sciences campus, kochi, kerala, india. It was estimated at the end of the 20th century that more than two billion people globally 34% of the worlds population will rely on medicinal plants as their. Comparative gcms analysis of bioactive compounds in. Gcms analysis and preliminary antimicrobial activity of. Gc ms analysis methanol extract of solanum torvum leaves was analyzed with the help of gc ms analyzer perkin. Abstract the traditional medicine still plays an important role in the primary health care in yemen. Gcms analysis of volatile constituents some extracted. Gc ms analysis gc ms was performed using the gc clarus 500 perkin elmer equipment. Screening of bioactive components of the flower datura metel. Here we report the presence of some important compounds in this plant isolated by gc ms analysis. Natural remedies from medicinal plants prove as safe and effective, as many plant species have been used in folklore medicine to treat various ailments. Recently gcms analysis is increasingly applied for the. The gcms analysis of bioactive compounds from the different extracts of the leaves of b. Gcms analysis was carried out on a perkin elmer turbo mass spectrophotometer norwalk, cto6859, and usa which includes a perkin elmer auto sampler xlgc.
Phytochemical, cordia dichotoma leaves, gc ms analysis introduction the search for natural products to cure diseases represents an area of great interest in which plants have been the most important source. Medicinal plants have had a crucial role in human culture and civilization. Gcms analysis gcms was performed using the gc clarus 500 perkin elmer equipment. Gc ms analysis has been found to be an ideal technique for the analysis of volatile and semivolatile bioactive compounds16. In recent years gcms studies have been increasingly applied for the analysis of medicinal plants as this technique has proved to be a valuable method for the analysis of non polar components and. Analysis of herbicidal activity from medicinal plants by. Weeds are one of the major problems in agriculture that results in reduction of crop yields, especially grasses, vegetable and fruit plants. Phytochemical analysis by gcms analysis of the plant rhizomes revealed the presence of different fatty acids, heterocyclic compounds etc. Gcms analysis of phytocomponents in the ethanol extract. These extracts contain 37 chemical components which are monoterpenes, steroids precursor and fatty acids.
Screening of bioactive components of the flower datura. The fatty acids detected in the salsola vermiculata and suaeda vermiculata extracts are known to possess several biological activities. Gcms analysis of bioactive compounds in methanolic extract of. Gas chromatographymass spectrometry gcms analysis of. There are numerous reports on the gc ms analysis studies. Gcms analysis of ethanolic extract of heliotropium indicum leaves revealed the existence of squalene 23. The whole plant of rostellularia diffusa was extracted with ethyl acetate at room temperature for 72 h.
Volatile compounds are identified by the gcms analysis3. Study on phytochemical screening, hplc analysis of phenols. Preliminary phytochemical screening and gc ms issn. Preliminary phytochemical screening and gc ms issn profiling. The chemical compositions of methanol extract of plant. Marine science and biological research authority, aden, p. The rajas college autonomous, pudukkottai 622 001, tamil nadu, india. Gcms analysis were performed using a perkinelmer gc clauses 500 system and gas chromatograph interfaced to a mass spectrometer gcms equipped with a elite1, fused silica capillary column 30 m. Qualitative analysis, total phenolic content, ftir and gc ms characterisation of canna indica. Principle and application of gcms detector in phytochemical analysis gcms plays a key role in the analysis of unknown components of plant origin. Phytochemical screening and gcms analysis of leaf extract. Phytochemical, gcms analysis and antibacterial activity.
Lcmsms and gcms analyses of biologically active extracts and fractions from tunisian juniperus phoenice leaves henda keskesa, sahla belhadjb, lobna jlailc. Gcms analysis has been found to be an ideal technique for the analysis of volatile and semivolatile bioactive compounds16. Gc ms ionizes compounds and measures their mass numbers. In recent years gcms studies have been increasingly applied for the analysis of chinese medicinal plants as this technique has proved corresponding author purushoth prabhu. Gcms analysis of chloroform extract of cassia fistula leaves retention time name of the compound molecular weight molecular formula 15. The gcms analysis of the ethanolic extract revealed the presence of 14 major compounds. Phytochemical analysis of hybanthus enneaspermus using uv.
To identify the various phyto constituents present in the unexplored plant rostellularia diffusa willd. Gc ms analysis of chemical compounds from some yemeni medicinal plants nabil qaid m. Gcms analysis, phytoconstituents, zingiber roseum, curcuma angustifolia, globba marantina. International journal of environmental analytical chemistry. Research article using ftir, hplc and gcms analysis.
The fatty acids detected in the salsola vermiculata and suaeda vermiculata extracts are known to possess several biological activities table 4. Phytochemical screening and gcms analysis of leaf extract of. The name, molecular weight and structure of the components of the test materials were ascertained. A study of phytochemical constituents in caralluma. Pdf the gc ms analysis of one medicinal plant, premna. Aim of this study is the gas chromatographymass spectroscopy analysis of chemical constituents of chenopodium murale leaves in two different extracts.
Pdf gcms analysis of chemical compounds from some yemeni. Gcms analysis were performed an a shimadzu gcms qp2010a system in et mode 70ev equipped with a split splitters injector 250. Gcms analysis of the methanol extract of eclipta prostrata was performed using a perkinelmer gc clarus 500 system comprising an aoc20i autosampler and a gas chromatograph interfaced to a mass spectrometer gcms equipped with a elite5ms 5% diphenyl95% dimethyl poly siloxane fused a capillary column 30. Gcms analysis of the ethanolic extract of the whole. The medicinal value of plants lies in some chemical substances that produce a definite physiological action on the human body. Academic sciences asian journal of pharmaceutical and. With this background the present study was aimed to identify the phytoconstituents in indigofera suffruticosa leaves by using gc ms analysis.
Chandra mohan and sruthi dinakar and thangavel anand and rasokkiyam elayaraja and b. Nov 27, 2019 aim of this study is the gas chromatographymass spectroscopy analysis of chemical constituents of chenopodium murale leaves in two different extracts. It were dried at room temperature 30c for a period of 14 days and made into powder using homogizer. A large number of medicinal plants and their purified constituents have shown beneficial therapeutic potentials. Gcms analysis of the whole plant ethanol extract of p. Gcms analysis of the ethanolic extract of the whole plant. Preliminary phytochemical screening and gc ms analysis of. Principle and application of gc ms detector in phytochemical analysis gc ms plays a key role in the analysis of unknown components of plant origin.
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